1S00

PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG M233 REPLACED WITH CYS IN THE CHARGE-SEPARATED D+QAQB- STATE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	L, R	Reaction center protein L chain	polymer	281	31345.4	2	UniProt (P02954) Pfam (PF00124) UniProt (by SIFTS) (P0C0Y8) In PDB	Rhodobacter sphaeroides	Photosynthetic reaction center L subunit
2	M, S	Reaction center protein M chain	polymer	307	34344.5	2	UniProt (P02953) Pfam (PF00124) UniProt (by SIFTS) (P0C0Y9) In PDB	Rhodobacter sphaeroides	Photosynthetic reaction center M subunit
3	H, T	Reaction center protein H chain	polymer	260	28066.3	2	UniProt (P11846) Pfam (PF03967) Pfam (PF05239) UniProt (by SIFTS) (P0C0Y7) In PDB	Rhodobacter sphaeroides	Photosynthetic reaction center H subunit
4	L, M, R, S	BACTERIOCHLOROPHYLL A	non-polymer		911.5	8	Chemie (BCL)
5	L, M, R, S	BACTERIOPHEOPHYTIN A	non-polymer		889.2	4	Chemie (BPH)
6	L, M, R, S	UBIQUINONE-10	non-polymer		863.3	4	Chemie (U10)
7	M, S	FE (II) ION	non-polymer		55.8	2	Chemie (FE2)
8	S, M	SPHEROIDENE	non-polymer		568.9	2	Chemie (SPO)
9	M	LAURYL DIMETHYLAMINE-N-OXIDE	non-polymer		229.4	1	Chemie (LDA)
10		water	water		18.0	156	Chemie (HOH)

Sequence modifications

L, R: 1 - 281 (UniProt: P02954)

PDB	External Database	Details
Asn 213	Asp 213	engineered mutation

M, S: 1 - 307 (UniProt: P02953)

PDB	External Database	Details
Cys 233	Arg 233	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	187512.4
Non-Polymers*	Number of molecules	21
	Total formula weight	15781.2
All*	Total formula weight	203293.6

*Water molecules are not included.