1R30
The Crystal Structure of Biotin Synthase, an S-Adenosylmethionine-Dependent Radical Enzyme
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Biotin synthase | polymer | 369 | 41362.7 | 2 | UniProt (P12996) Pfam (PF04055) Pfam (PF06968) | Escherichia coli | Biotin synthetase |
| 2 | C, H (A, B) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 2 | Chemie (SAM) | |||
| 3 | D, I (A, B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 4 | E, J (A, B) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 5 | F, K (A, B) | 6-(5-METHYL-2-OXO-IMIDAZOLIDIN-4-YL)-HEXANOIC ACID | non-polymer | 214.3 | 2 | Chemie (DTB) | |||
| 6 | G (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) |
Sequence modifications
A, B: 2 - 346 (UniProt: P12996)
| PDB | External Database | Details |
|---|---|---|
| Gly -22 | - | expression tag |
| Ser -21 | - | expression tag |
| Ser -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| Asp -13 | - | linker |
| Tyr -12 | - | linker |
| Asp -11 | - | linker |
| Ile -10 | - | linker |
| Pro -9 | - | linker |
| Thr -8 | - | linker |
| Thr -7 | - | linker |
| Glu -6 | - | linker |
| Asn -5 | - | linker |
| Leu -4 | - | linker |
| Tyr -3 | - | linker |
| Phe -2 | - | linker |
| Gln -1 | - | linker |
| Gly 0 | - | linker |
| Ser 1 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 82725.4 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2402.5 | |
| All* | Total formula weight | 85127.9 |
