1R30
The Crystal Structure of Biotin Synthase, an S-Adenosylmethionine-Dependent Radical Enzyme
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Biotin synthase | polymer | 369 | 41362.7 | 2 | UniProt (P12996) Pfam (PF04055) Pfam (PF06968) | Escherichia coli | Biotin synthetase |
| 2 | C, H (A, B) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 2 | Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (9865603) PubChem (145712080) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094) | |||
| 3 | D, I (A, B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 4 | E, J (A, B) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 5 | F, K (A, B) | 6-(5-METHYL-2-OXO-IMIDAZOLIDIN-4-YL)-HEXANOIC ACID | non-polymer | 214.3 | 2 | Chemie (DTB) PubChem (643) PubChem (36688066) PubChem (59583706) PubChem (445027) PubChem (70834907) PubChem (11966269) PubChem (11966270) | |||
| 6 | G (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) PubChem (3777159) |
Sequence modifications
A, B: 2 - 346 (UniProt: P12996)
| PDB | External Database | Details |
|---|---|---|
| Gly -22 | - | expression tag |
| Ser -21 | - | expression tag |
| Ser -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| Asp -13 | - | linker |
| Tyr -12 | - | linker |
| Asp -11 | - | linker |
| Ile -10 | - | linker |
| Pro -9 | - | linker |
| Thr -8 | - | linker |
| Thr -7 | - | linker |
| Glu -6 | - | linker |
| Asn -5 | - | linker |
| Leu -4 | - | linker |
| Tyr -3 | - | linker |
| Phe -2 | - | linker |
| Gln -1 | - | linker |
| Gly 0 | - | linker |
| Ser 1 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 82725.4 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2402.5 | |
| All* | Total formula weight | 85127.9 |
