1PZY

W314A-BETA1,4-GALACTOSYLTRANSFERASE-I COMPLEXED WITH ALPHA-LACTALBUMIN IN THE PRESENCE OF N-ACETYLGLUCOSAMINE, UDP AND MANGANESE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	ALPHA-LACTALBUMIN	polymer	123	14015.8	2	UniProt (P29752) Pfam (PF00062)	Mus musculus (house mouse)	lactalbumin, alpha
2	B, D (B, D)	BETA-1,4-GALACTOSYLTRANSFERASE	polymer	286	32734.4	2	UniProt (P08037) Pfam (PF13733) Pfam (PF02709)	Bos taurus (cattle)	B4gal-t1; BETA4GAL-T1; BETA-1,4-GALTASE 1; BETA-1,4-GALACTOSYLTRANSFERASE 1; UDP-GALACTOSE:BETA-N-ACETYLGLUCOSAMINE BETA-1,4-GALACTOSYLTRANSFERASE 1
3	E, I (A, C)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	F, J (B, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5	G, K (B, D)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
6	H, L (B, D)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP)
7	M, N, O, P (A, B, C, D)	water	water		18.0	251	Chemie (HOH)

Sequence modifications

B, D: 130 - 402 (UniProt: P08037)

PDB	External Database	Details
Ala 117	-	expression tag
Ser 118	-	expression tag
Met 119	-	expression tag
Thr 120	-	expression tag
Gly 121	-	expression tag
Gly 122	-	expression tag
Gln 123	-	expression tag
Gln 124	-	expression tag
Met 125	-	expression tag
Gly 126	-	expression tag
Arg 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
Ala 314	Trp 241	engineered mutation
Thr 342	Cys 269	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	93500.6
Non-Polymers*	Number of molecules	8
	Total formula weight	1440.8
All*	Total formula weight	94941.3

*Water molecules are not included.