1PVP

BASIS FOR A SWITCH IN SUBSTRATE SPECIFICITY: CRYSTAL STRUCTURE OF SELECTED VARIANT OF CRE SITE-SPECIFIC RECOMBINASE, ALSHG BOUND TO THE ENGINEERED RECOGNITION SITE LOXM7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	34-MER	polymer	34	10469.8	1	GenBank (M10145)	Escherichia virus P1
2	D	34-MER	polymer	34	10438.8	1	GenBank (M10494)	Escherichia virus P1
3	A, B	Recombinase cre	polymer	349	39260.9	2	UniProt (P06956) Pfam (PF00589) In PDB	Escherichia phage P1 (Bacteriophage P1)
4		water	water		18.0	256	Chemie (HOH)

Sequence modifications

C: 1 - 34 (GenBank: M10145)

PDB	External Database	Details
Dc 7	Dt 30	engineered mutation
Dt 8	Dc 31	engineered mutation
Da 9	Dg 32	engineered mutation
Dt 26	Dc 49	engineered mutation
Da 27	Dg 50	engineered mutation
Dg 28	Da 51	engineered mutation

D: 1 - 34 (GenBank: M10494)

PDB	External Database	Details
Dc 7	Dt 20	engineered mutation
Dt 8	Dc 21	engineered mutation
Da 9	Dg 22	engineered mutation
Dt 26	Dc 39	engineered mutation
Da 27	Dg 40	engineered mutation
Dg 28	Da 41	engineered mutation

A, B: 2 - 343 (UniProt: P06956)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Ala 174	Ile 174	engineered mutation
Leu 258	Thr 258	engineered mutation
Ser 259	Arg 259	engineered mutation
His 262	Glu 262	engineered mutation
Gly 266	Glu 266	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	99430.3
All*	Total formula weight	99430.3

*Water molecules are not included.