1P4E

Flpe W330F mutant-DNA Holliday Junction Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (E, F)	5'-D(*TP*AP*AP*GP*TP*TP*CP*CP*TP*AP*TP*TP*C)-3'	polymer	13	3916.6	2
2	B, D (I, J)	5'-D(*TP*TP*TP*AP*AP*AP*AP*GP*AP*AP*TP*AP*GP*GP*AP*AP*CP*TP*TP*C)-3'	polymer	20	6165.0	2
3	E, F (G, H)	33-MER	polymer	33	10126.6	2
4	G, H (A, B)	Recombinase FLP protein	polymer	429	49383.4	2	UniProt (P03870) Pfam (PF03930) Pfam (PF05202)	Saccharomyces cerevisiae (baker's yeast)	Protein Able
5	I, J (C, D)	Recombinase FLP protein	polymer	429	49463.4	2	UniProt (P03870) Pfam (PF03930) Pfam (PF05202)	Saccharomyces cerevisiae (baker's yeast)	Protein Able
6	K (H)	PHOSPHONATE	non-polymer		80.0	1	Chemie (2PO)
7	L, M, N, O, P... (I, F, J, G, H...)	water	water		18.0	266	Chemie (HOH)

Sequence modifications

A, B: 2 - 423 (UniProt: P03870)

PDB	External Database	Details
Ser 2	Pro 3	SEE REMARK 999
Asp 5	Gly 6	variant
Ser 33	Leu 34	SEE REMARK 999
Asn 108	Tyr 109	SEE REMARK 999
Pro 294	Ser 295	SEE REMARK 999
Phe 330	Trp 331	engineered mutation
Gly 424	-	expression tag
His 425	-	expression tag
His 426	-	expression tag
His 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag

C, D: 2 - 422 (UniProt: P03870)

PDB	External Database	Details
Ser 2	Pro 3	SEE REMARK 999
Asp 5	Gly 6	variant
Ser 33	Leu 34	SEE REMARK 999
Asn 108	Tyr 109	SEE REMARK 999
Pro 294	Ser 295	SEE REMARK 999
Phe 330	Trp 331	engineered mutation
Gly 424	-	expression tag
His 425	-	expression tag
His 426	-	expression tag
His 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	238109.9
Non-Polymers*	Number of molecules	1
	Total formula weight	80.0
All*	Total formula weight	238189.9

*Water molecules are not included.