1P16
Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | mRNA capping enzyme alpha subunit | polymer | 395 | 46496.7 | 2 | UniProt (P78587) | Candida albicans | mRNA guanylyltransferase; GTP--RNA guanylyltransferase; GTase |
| 2 | C, D (C, D) | phosphorylated peptide from C-terminal of RNA polymerase II | polymer | 21 | 2417.2 | 2 | phosphorylated CTD peptide | ||
| 3 | E, F, G (A) | PHOSPHATE ION | non-polymer | 95.0 | 3 | Chemie (PO4) PubChem (1061) | |||
| 4 | H (A) | GUANOSINE-5'-MONOPHOSPHATE | non-polymer | 363.2 | 1 | Chemie (5GP) | |||
| 5 | I (B) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 6 | J, K, L, M (A, B, C, D) | water | water | 18.0 | 433 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 395 (UniProt: P78587)
| PDB | External Database | Details |
|---|---|---|
| Mse 1 | Met 1 | cloning artifact |
| Val 2 | Ile 2 | engineered mutation |
| Mse 28 | Met 28 | cloning artifact |
| Mse 58 | Met 58 | cloning artifact |
| Mse 171 | Met 171 | cloning artifact |
| Mse 199 | Met 199 | cloning artifact |
| Mse 212 | Met 212 | cloning artifact |
| Mse 338 | Met 338 | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 97827.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1171.3 | |
| All* | Total formula weight | 98999.0 |
