1OZG

The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Acetolactate synthase, catabolic	polymer	566	61355.8	2	UniProt (P27696) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775) In PDB	Klebsiella pneumoniae	ALS
2	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, B	2-HYDROXYETHYL DIHYDROTHIACHROME DIPHOSPHATE	non-polymer		468.4	2	Chemie (HE3)
5	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	8	Chemie (PEG)
6		water	water		18.0	612	Chemie (HOH)

Sequence modifications

A, B: 1 - 566 (UniProt: P27696)

PDB	External Database	Details
Glu 560	-	expression tag
His 561	-	expression tag
His 562	-	expression tag
His 563	-	expression tag
His 564	-	expression tag
His 565	-	expression tag
His 566	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	122711.5
Non-Polymers*	Number of molecules	14
	Total formula weight	2024.2
All*	Total formula weight	124735.8

*Water molecules are not included.