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Crystal structure of the periplasmic endonuclease Vvn complexed with octamer double stranded DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E (C, D, F)	5'-D(*GP*CP*GP*AP*TP*CP*GP*C)-3'	polymer	8	2427.6	3
2	C (E)	5'-D(*GP*CP*GP*AP*TP*C)-3'	polymer	6	1809.2	1
3	D (G)	5'-D(P*GP*C)-3'	polymer	2	573.4	1
4	F, G (A, B)	Nuclease	polymer	213	24751.7	2	UniProt (Q8DCA6) Pfam (PF04231) UniProt (by SIFTS) (Q7MHK3)	Vibrio vulnificus	VVN Nuclease
5	H, I (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	J, K, L, M, N... (C, D, E, G, F...)	water	water		18.0	328	Chemie (HOH)

Sequence modifications

A, B: 19 - 231 (UniProt: Q8DCA6)

PDB	External Database	Details
Ala 80	His 80	engineered mutation
Pro 163	Gln 163	SEE REMARK 999
Asp 164	Thr 164	SEE REMARK 999
Arg 165	Glu 165	SEE REMARK 999
Ala 166	Leu 166	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	59168.9
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	59249.0

*Water molecules are not included.