1OR0

Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Glutaryl 7-Aminocephalosporanic Acid Acylase	polymer	160	17644.2	2	UniProt (Q84I62) UniProt (by SIFTS) (P07662)	Pseudomonas sp. SY-77-1
2	B, D (B, D)	glutaryl acylase	polymer	528	59624.6	2	UniProt (Q84I62) Pfam (PF01804) UniProt (by SIFTS) (P07662)	Pseudomonas sp. SY-77-1	Glutaryl 7-Aminocephalosporanic Acid Acylase
3	E, F (B, D)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
4	G, H, I, J (A, B, C, D)	water	water		18.0	1429	Chemie (HOH)

Sequence modifications

A, C: 1 - 160 (UniProt: Q84I62)

PDB	External Database	Details
Asp 125	Glu 154	SEE REMARK 999
Mse 145	Met 174	modified residue

B, D: 1 - 522 (UniProt: Q84I62)

PDB	External Database	Details
Mse 64	Met 262	modified residue
Mse 73	Met 271	modified residue
Mse 149	Met 347	modified residue
Mse 156	Met 354	modified residue
Mse 172	Met 370	modified residue
Mse 282	Met 480	modified residue
Mse 294	Met 492	modified residue
Mse 304	Met 502	modified residue
Mse 416	Met 614	modified residue
Mse 460	Met 658	modified residue
Mse 473	Met 671	modified residue
His 523	-	expression tag
His 524	-	expression tag
His 525	-	expression tag
His 526	-	expression tag
His 527	-	expression tag
His 528	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	154537.5
Non-Polymers*	Number of molecules	2
	Total formula weight	124.1
All*	Total formula weight	154661.7

*Water molecules are not included.