1OJK
Anatomy of glycosynthesis: Structure and kinetics of the Humicola insolens Cel7BE197A and E197S glycosynthase mutants
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | ENDOGLUCANASE I | polymer | 402 | 44568.1 | 2 | UniProt (P56680) Pfam (PF00840) | HUMICOLA INSOLENS | ENDO-1,4-BETA-GLUCANASE |
| 2 | C, E (C, E) | beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900023) GlyTouCan (G86802XL) | alpha-cellobiose | ||
| 3 | D, F (D, F) | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900005) GlyTouCan (G84824ZO) | beta-cellobiose | ||
| 4 | G, I (A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 5 | H, J (A, B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 6 | K, L (A, B) | water | water | 18.0 | 793 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 402 (UniProt: P56680)
| PDB | External Database | Details |
|---|---|---|
| Ser 197 | Glu 197 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 89136.2 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1369.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 626.6 | |
| All* | Total formula weight | 91132.0 |
