1OI8

5'-Nucleotidase (E. coli) with an Engineered Disulfide Bridge (P90C, L424C)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	PROTEIN USHA	polymer	532	59231.6	2	UniProt (P07024) Pfam (PF00149) Pfam (PF02872) In PDB	ESCHERICHIA COLI	5-NUCLEOTIDASE
2	A, B	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
3	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	A, B	CARBONATE ION	non-polymer		60.0	2	Chemie (CO3)
5		water	water		18.0	916	Chemie (HOH)

Sequence modifications

A, B: 26 - 550 (UniProt: P07024)

PDB	External Database	Details
Cys 90	Pro 90	engineered mutation
Cys 424	Leu 424	engineered mutation

A, B: 551 - 557 (PDB: 1OI8)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	118463.3
Non-Polymers*	Number of molecules	8
	Total formula weight	531.9
All*	Total formula weight	118995.1

*Water molecules are not included.