1OHS

CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANT Y14F/D38N FROM PSEUDOMONAS TESTOSTERONI COMPLEXED WITH ANDROSTANEDIONE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	STEROID DELTA-ISOMERASE	polymer	125	13407.2	4	UniProt (P00947) Pfam (PF12680)	PSEUDOMONAS TESTOSTERONI	DELTA-5-3-KETOSTEROID ISOMERASE, KETOSTEROID ISOMERASE
2	E, F, G (A, B, C)	5ALPHA-ANDROSTAN-3,17-DIONE	non-polymer		288.4	3	Chemie (5SD)
3	H, I, J, K (A, B, C, D)	water	water		18.0	419	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 125 (UniProt: P00947)

PDB	External Database	Details
Phe 14	Tyr 14	engineered mutation
Asn 38	Asp 38	engineered mutation
Ile 83	Thr 83	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	53628.7
Non-Polymers*	Number of molecules	3
	Total formula weight	865.3
All*	Total formula weight	54494.0

*Water molecules are not included.