1NZK

Crystal Structure of a Multiple Mutant (L44F, L73V, V109L, L111I, C117V) of Human Acidic Fibroblast Growth Factor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Acidic Fibroblast Growth Factor	polymer	143	16427.5	4	UniProt (P05230) Pfam (PF00167)	Homo sapiens (human)	Heparin-binding growth factor 1; HBGF-1; AFGF; Beta-endothelial cell growth factor; ECGF-beta
2	E, H, K, N (A, B, C, D)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	F, G, I, J, L... (A, B, C, D)	FORMIC ACID	non-polymer		46.0	8	Chemie (FMT)
4	Q, R, S, T (A, B, C, D)	water	water		18.0	437	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 137 (UniProt: P05230)

PDB	External Database	Details
His 1	-	expression tag
His 1	-	expression tag
His 1	-	expression tag
His 1	-	expression tag
His 1	-	expression tag
His 1	-	expression tag
Phe 44	Leu 59	engineered mutation
Val 73	Leu 88	engineered mutation
Leu 109	Val 124	engineered mutation
Ile 111	Leu 126	engineered mutation
Val 117	Cys 132	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	65710.0
Non-Polymers*	Number of molecules	12
	Total formula weight	752.5
All*	Total formula weight	66462.5

*Water molecules are not included.