1NS2

Crystal structure of galactose mutarotase from Lactococcus lactis mutant E304A complexed with galactose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	GALACTOSE MUTAROTASE	polymer	347	38564.9	2	UniProt (Q9ZB17) Pfam (PF01263) In PDB	Lactococcus lactis	ALDOSE 1-EPIMERASE
2	A, B	beta-D-galactopyranose	non-polymer		180.2	2	Chemie (GAL)
3	A	NICKEL (II) ION	non-polymer		58.7	1	Chemie (NI)
4		water	water		18.0	402	Chemie (HOH)

Sequence modifications

A, B: 1 - 339 (UniProt: Q9ZB17)

PDB	External Database	Details
Ser 2	Glu 2	cloning artifact
Ala 304	Glu 304	engineered mutation
Leu 340	-	expression tag
Glu 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	77129.8
Non-Polymers*	Number of molecules	3
	Total formula weight	419.0
All*	Total formula weight	77548.8

*Water molecules are not included.