1NI0

Structure of the Y94F mutant of the restriction endonuclease PvuII

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Type II restriction enzyme PvuII	polymer	159	18497.1	3	UniProt (P23657) Pfam (PF09225)	Proteus vulgaris	Endonuclease PvuII, R.PvuII
2	D, E, F (A, B, C)	water	water		18.0	187	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 157 (UniProt: P23657)

PDB	External Database	Details
Phe 94	Tyr 94	engineered mutation
Ala 158	-	expression tag
Ala 159	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	55491.4
All*	Total formula weight	55491.4

*Water molecules are not included.