1MVY
Amylosucrase mutant E328Q co-crystallized with maltoheptaose.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | amylosucrase | polymer | 628 | 71530.1 | 1 | GenBank (4107260) Pfam (PF00128) Pfam (PF22582) UniProt (by SIFTS) (Q9ZEU2) | Neisseria polysaccharea | |
| 2 | B (B) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 666.6 | 1 | In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ) | alpha-maltotetraose | ||
| 3 | C (C) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | D (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 5 | E (A) | water | water | 18.0 | 465 | Chemie (HOH) |
Sequence modifications
A: 5 - 628 (GenBank: 4107260)
| PDB | External Database | Details |
|---|---|---|
| Ser 1 | - | cloning artifact |
| Pro 2 | - | cloning artifact |
| Asn 3 | - | cloning artifact |
| Ser 4 | - | cloning artifact |
| Tyr 131 | His 139 | SEE REMARK 999 |
| Gln 328 | Glu 336 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 71530.1 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1008.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 122.1 | |
| All* | Total formula weight | 72661.1 |
