1MUS
crystal structure of Tn5 transposase complexed with resolved outside end DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | DNA transferred strand | polymer | 20 | 6213.0 | 1 | |||
| 2 | B (C) | DNA non-transferred strand | polymer | 20 | 6052.9 | 1 | |||
| 3 | C (A) | Tn5 transposase | polymer | 477 | 53323.1 | 1 | UniProt (Q46731) Pfam (PF14706) Pfam (PF01609) | Escherichia coli | |
| 4 | D, E (A) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 5 | F, G (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | H, I, J (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 7 | K, L, M (B, C, A) | water | water | 18.0 | 615 | Chemie (HOH) |
Sequence modifications
A: 1 - 476 (UniProt: Q46731)
| PDB | External Database | Details |
|---|---|---|
| Lys 54 | Glu 54 | engineered mutation |
| Ala 56 | Met 56 | engineered mutation |
| Lys 119 | Asp 119 | engineered mutation |
| Ala 120 | Lys 120 | engineered mutation |
| Lys 345 | Glu 345 | engineered mutation |
| Pro 372 | Leu 372 | engineered mutation |
| Gly 477 | - | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 65589.1 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 344.7 | |
| All* | Total formula weight | 65933.8 |
