1MRD
PREPARATION, CHARACTERIZATION AND CRYSTALLIZATION OF AN ANTIBODY FAB FRAGMENT THAT RECOGNIZES RNA. CRYSTAL STRUCTURES OF NATIVE FAB AND THREE FAB-MONONUCLEOTIDE COMPLEXES
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (L) | IGG2B-KAPPA JEL103 FAB (LIGHT CHAIN) | polymer | 219 | 24180.9 | 1 | PIR (PC4203) | Mus musculus (house mouse) | |
| 2 | B (H) | IGG2B-KAPPA JEL103 FAB (HEAVY CHAIN) | polymer | 215 | 23020.6 | 1 | GenBank (387221) | Mus musculus (house mouse) | |
| 3 | C (L) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 4 | D (L) | IMIDAZOLE | non-polymer | 69.1 | 1 | Chemie (IMD) | |||
| 5 | E (L) | INOSINE-5'-DIPHOSPHATE | non-polymer | 428.2 | 1 | Chemie (IDP) PubChem (136053441) PubChem (135398651) PubChem (135402347) PubChem (135450593) PubChem (135887991) PubChem (136183346) PubChem (136269906) PubChem (136364699) PubChem (136706925) PubChem (136706926) PubChem (136706927) PubChem (137031712) PubChem (137083597) PubChem (137137131) PubChem (137256750) PubChem (162468660) PubChem (166055571) PubChem (173972243) | |||
| 6 | F, G (L, H) | water | water | 18.0 | 103 | Chemie (HOH) |
Sequence modifications
L: 1 - 214 (PIR: PC4203)
H: 2 - 227 (GenBank: 387221)
| PDB | External Database | Details |
|---|---|---|
| Val 3 | Leu 3 | conflict |
| Leu 27 | Ile 29 | conflict |
| Ser 27 | Thr 32 | conflict |
| His 34 | Glu 39 | conflict |
| Phe 87 | Tyr 92 | conflict |
| Ser 89 | Phe 94 | conflict |
| Ser 91 | Gly 96 | conflict |
| Thr 92 | Ser 97 | conflict |
| Lys 153 | Ser 158 | conflict |
| Asn 167 | Asp 172 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Lys 13 | Arg 13 | conflict |
| Ala 16 | Thr 16 | conflict |
| Leu 20 | Met 20 | conflict |
| Ser 25 | Ala 25 | conflict |
| Ser 31 | Asn 31 | conflict |
| Met 34 | Ile 34 | conflict |
| Gln 35 | Gly 35 | conflict |
| Gln 39 | Glu 39 | conflict |
| Gln 43 | His 43 | conflict |
| Glu 50 | Asp 50 | conflict |
| Asp 52 | Tyr 52 | conflict |
| Ser 53 | Gly 54 | conflict |
| Asp 54 | Gly 55 | conflict |
| Ser 55 | Gly 56 | conflict |
| Tyr 56 | Phe 57 | conflict |
| Gln 61 | Glu 62 | conflict |
| Lys 62 | Asn 63 | conflict |
| Val 71 | Ala 72 | conflict |
| Val 89 | Ile 93 | conflict |
| Tyr 91 | His 95 | conflict |
| - | Arg 98 | deletion |
| - | Gly 99 | deletion |
| - | Ile 100 | deletion |
| - | Tyr 101 | deletion |
| - | Tyr 102 | deletion |
| Leu 95 | Ser 104 | conflict |
| Arg 96 | Ser 105 | conflict |
| Gly 97 | Pro 106 | conflict |
| Tyr 102 | Ser 110 | conflict |
| Leu 140 | Ser 146 | conflict |
| Ser 151 | Pro 157 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 47201.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 562.7 | |
| All* | Total formula weight | 47764.1 |
