1MM8
Crystal structure of Tn5 Transposase complexed with ME DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | ME DNA transferred strand | polymer | 20 | 6271.1 | 1 | |||
| 2 | B (C) | ME DNA non-transferred strand | polymer | 20 | 5994.9 | 1 | |||
| 3 | C (A) | Tn5 Transposase | polymer | 481 | 53845.7 | 1 | UniProt (Q46731) Pfam (PF14706) Pfam (PF01609) | Escherichia coli | |
| 4 | D, E (A) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 5 | F, G, H (B, C, A) | water | water | 18.0 | 254 | Chemie (HOH) |
Sequence modifications
A: -3 - 476 (UniProt: Q46731)
| PDB | External Database | Details |
|---|---|---|
| Lys 54 | Glu 58 | engineered mutation |
| Ala 56 | Met 60 | engineered mutation |
| Lys 345 | Glu 349 | engineered mutation |
| Pro 372 | Leu 376 | engineered mutation |
| Gly 477 | - | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 66111.7 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 109.9 | |
| All* | Total formula weight | 66221.6 |
