1MM8
Crystal structure of Tn5 Transposase complexed with ME DNA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | B | ME DNA transferred strand | polymer | 20 | 6271.1 | 1 | |||
2 | C | ME DNA non-transferred strand | polymer | 20 | 5994.9 | 1 | |||
3 | A | Tn5 Transposase | polymer | 481 | 53845.7 | 1 | UniProt (Q46731) Pfam (PF14706) Pfam (PF01609) In PDB | Escherichia coli | |
4 | A | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
5 | water | water | 18.0 | 254 | Chemie (HOH) |
Sequence modifications
A: -3 - 476 (UniProt: Q46731)
PDB | External Database | Details |
---|---|---|
Lys 54 | Glu 58 | engineered mutation |
Ala 56 | Met 60 | engineered mutation |
Lys 345 | Glu 349 | engineered mutation |
Pro 372 | Leu 376 | engineered mutation |
Gly 477 | - | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 66111.7 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 109.9 | |
All* | Total formula weight | 66221.6 |