1MI0

Crystal Structure of the redesigned protein G variant NuG2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	immunoglobulin-binding protein G	polymer	65	7356.0	2	PIR (A45063) Pfam (PF01378) UniProt (by SIFTS) (Q53291)	Finegoldia magna
2	C, D (A, B)	water	water		18.0	110	Chemie (HOH)

Sequence modifications

A, B: 5 - 61 (PIR: A45063)

PDB	External Database	Details
Met -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
Ala 4	-	expression tag
Val 11	Ile 334	SEE REMARK 999
Ile 12	Leu 335	SEE REMARK 999
Val 13	Asn 336	SEE REMARK 999
Leu 14	Gly 337	SEE REMARK 999
Asn 15	Lys 338	SEE REMARK 999
Gly 16	Thr 339	SEE REMARK 999
Thr 17	Leu 340	SEE REMARK 999
Thr 18	Lys 341	SEE REMARK 999
Phe 19	Gly 342	SEE REMARK 999
Thr 20	Glu 343	SEE REMARK 999
Tyr 21	Thr 344	SEE REMARK 999
Ala 51	Asp 374	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	14712.1
All*	Total formula weight	14712.1

*Water molecules are not included.