1MEP
Crystal Structure of Streptavidin Double Mutant S45A/D128A with Biotin: Cooperative Hydrogen-Bond Interactions in the Streptavidin-Biotin System.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Streptavidin | polymer | 127 | 13221.3 | 4 | UniProt (P22629) Pfam (PF01382) | Streptomyces avidinii | |
| 2 | E, F, G, H (A, B, C, D) | BIOTIN | non-polymer | 244.3 | 4 | Chemie (BTN) | |||
| 3 | I, J, K, L (A, B, C, D) | water | water | 18.0 | 293 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 13 - 139 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Ala 45 | Ser 69 | engineered mutation |
| Ala 128 | Asp 152 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 52885.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 977.2 | |
| All* | Total formula weight | 53862.5 |
