1M9H
Corynebacterium 2,5-DKGR A and Phe 22 replaced with Tyr (F22Y), Lys 232 replaced with Gly (K232G), Arg 238 replaced with His (R238H)and Ala 272 replaced with Gly (A272G)in presence of NADH cofactor
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 2,5-diketo-D-gluconic acid reductase A | polymer | 278 | 30062.5 | 1 | UniProt (P06632) Pfam (PF00248) | Corynebacterium sp. | 2,5-DKG reductase A, 2,5-DKGR A, 25DKGR-A, AKR5C |
| 2 | B (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 3 | C (A) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 1 | Chemie (NAD) | |||
| 4 | D (A) | water | water | 18.0 | 146 | Chemie (HOH) |
Sequence modifications
A: 1 - 278 (UniProt: P06632)
| PDB | External Database | Details |
|---|---|---|
| Tyr 22 | Phe 21 | engineered mutation |
| Gly 232 | Lys 231 | engineered mutation |
| His 238 | Arg 237 | engineered mutation |
| Gly 272 | Ala 271 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 30062.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 759.5 | |
| All* | Total formula weight | 30822.0 |
