1M9H
Corynebacterium 2,5-DKGR A and Phe 22 replaced with Tyr (F22Y), Lys 232 replaced with Gly (K232G), Arg 238 replaced with His (R238H)and Ala 272 replaced with Gly (A272G)in presence of NADH cofactor
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | 2,5-diketo-D-gluconic acid reductase A | polymer | 278 | 30062.5 | 1 | UniProt (P06632) Pfam (PF00248) In PDB | Corynebacterium sp. | 2,5-DKG reductase A, 2,5-DKGR A, 25DKGR-A, AKR5C |
2 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
3 | A | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 1 | Chemie (NAD) | |||
4 | water | water | 18.0 | 146 | Chemie (HOH) |
Sequence modifications
A: 1 - 278 (UniProt: P06632)
PDB | External Database | Details |
---|---|---|
Tyr 22 | Phe 21 | engineered mutation |
Gly 232 | Lys 231 | engineered mutation |
His 238 | Arg 237 | engineered mutation |
Gly 272 | Ala 271 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 30062.5 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 759.5 | |
All* | Total formula weight | 30822.0 |