1LWS
Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | PI-SceI DNA recognition region top strand | polymer | 37 | 11599.5 | 1 | |||
| 2 | B (C) | PI-SceI DNA recognition region bottom strand | polymer | 37 | 11172.2 | 1 | |||
| 3 | C (A) | ENDONUCLEASE PI-SCEI | polymer | 454 | 51379.1 | 1 | UniProt (P17255) Pfam (PF05203) Pfam (PF05204) | Saccharomyces cerevisiae (baker's yeast) | PI-SceI, VMA-derived endonuclease, VDE, SCE VMA intein |
| 4 | D, E (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) |
Sequence modifications
A: 1 - 454 (UniProt: P17255)
| PDB | External Database | Details |
|---|---|---|
| Mse 10 | Met 293 | modified residue |
| Mse 28 | Met 311 | modified residue |
| Mse 47 | Met 330 | modified residue |
| Mse 109 | Met 392 | modified residue |
| Mse 193 | Met 476 | modified residue |
| Mse 236 | Met 519 | modified residue |
| Mse 372 | Met 655 | modified residue |
| Mse 385 | Met 668 | modified residue |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 74150.7 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 80.2 | |
| All* | Total formula weight | 74230.9 |
