1KHL
E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N) COMPLEX WITH PHOSPHATE
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Alkaline Phosphatase | polymer | 449 | 47115.5 | 2 | UniProt (P00634) Pfam (PF00245) | Escherichia coli | |
| 2 | C, D, F, G (A, B) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 3 | E, H (A, B) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
| 4 | I, J (A, B) | water | water | 18.0 | 307 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 449 (UniProt: P00634)
| PDB | External Database | Details |
|---|---|---|
| Asn 15 | Asp 37 | conflict |
| Asn 35 | Asp 57 | conflict |
| His 153 | Asp 175 | engineered mutation |
| Gln 176 | Glu 198 | conflict |
| Glu 228 | Gln 250 | conflict |
| Glu 230 | Gln 252 | conflict |
| Asn 330 | Asp 352 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 94231.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 451.6 | |
| All* | Total formula weight | 94682.5 |
