1JYV
E. COLI (lacZ) BETA-GALACTOSIDASE (E537Q) IN COMPLEX WITH ONPG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Beta-Galactosidase | polymer | 1023 | 116505.3 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) | Escherichia coli | LACTASE; lacZ; beta-d-galactosidase |
| 2 | AC, BC, CC, E, F... (C, A, D, B) | MAGNESIUM ION | non-polymer | 24.3 | 17 | Chemie (MG) | |||
| 3 | DC, EC, FC, GC, J... (C, A, D, B) | SODIUM ION | non-polymer | 23.0 | 16 | Chemie (NA) | |||
| 4 | HC, IC, N, O, SD... (C, A, D, B) | 2-nitrophenyl beta-D-galactopyranoside | non-polymer | 301.2 | 8 | Chemie (145) | |||
| 5 | AA, AB, AD, AE, BA... (A, B, C, D) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 107 | Chemie (DMS) | |||
| 6 | WE, XE, YE, ZE (A, B, C, D) | water | water | 18.0 | 4081 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 1023 (UniProt: P00722)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | Thr 1 | cloning artifact |
| Ser 2 | Met 2 | cloning artifact |
| His 3 | Ile 3 | cloning artifact |
| Met 4 | Thr 4 | cloning artifact |
| Leu 5 | Asp 5 | cloning artifact |
| Glu 6 | Ser 6 | cloning artifact |
| Asp 7 | Leu 7 | cloning artifact |
| Pro 8 | Ala 8 | cloning artifact |
| Gln 537 | Glu 537 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 466021.1 | |
| Non-Polymers* | Number of molecules | 148 |
| Total formula weight | 11551.2 | |
| All* | Total formula weight | 477572.4 |
