1JML

Conversion of Monomeric Protein L to an Obligate Dimer by Computational Protein Design

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein L	polymer	72	8063.0	1	UniProt (Q51912) Pfam (PF02246)	Finegoldia magna	Ig light chain-binding protein
2	B, C, D (A)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
3	E (A)	water	water		18.0	39	Chemie (HOH)

Sequence modifications

A: 2 - 64 (UniProt: Q51912)

PDB	External Database	Details
Met -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Met 1	-	cloning artifact
Trp 47	Tyr 156	engineered mutation
Val 52	Ala 161	engineered mutation
Pro 53	Asp 162	engineered mutation
Ala 55	Gly 164	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	8063.0
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	8259.2

*Water molecules are not included.