1J3J
Double mutant (C59R+S108N) Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS) complexed with pyrimethamine, NADPH, and dUMP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Bifunctional dihydrofolate reductase-thymidylate synthase | polymer | 280 | 33214.3 | 2 | UniProt (P13922) Pfam (PF00186) | Plasmodium falciparum (malaria parasite P. falciparum) | |
| 2 | C, D (C, D) | Bifunctional dihydrofolate reductase-thymidylate synthase | polymer | 328 | 38712.9 | 2 | UniProt (P13922) Pfam (PF00303) | Plasmodium falciparum (malaria parasite P. falciparum) | |
| 3 | E, G (A, B) | 5-(4-CHLORO-PHENYL)-6-ETHYL-PYRIMIDINE-2,4-DIAMINE | non-polymer | 248.7 | 2 | Chemie (CP6) | |||
| 4 | F, H (A, B) | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 2 | Chemie (NDP) | |||
| 5 | I, J (C, D) | 2'-DEOXYURIDINE 5'-MONOPHOSPHATE | non-polymer | 308.2 | 2 | Chemie (UMP) | |||
| 6 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 733 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 280 (UniProt: P13922)
| PDB | External Database | Details |
|---|---|---|
| Arg 59 | Cys 59 | engineered mutation |
| Asn 108 | Ser 108 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 143854.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2604.6 | |
| All* | Total formula weight | 146459.0 |
