1I84

CRYO-EM STRUCTURE OF THE HEAVY MEROMYOSIN SUBFRAGMENT OF CHICKEN GIZZARD SMOOTH MUSCLE MYOSIN WITH REGULATORY LIGHT CHAIN IN THE DEPHOSPHORYLATED STATE. ONLY C ALPHAS PROVIDED FOR REGULATORY LIGHT CHAIN. ONLY BACKBONE ATOMS PROVIDED FOR S2 FRAGMENT.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	S, V	SMOOTH MUSCLE MYOSIN HEAVY CHAIN	polymer	1184	137072.9	2	UniProt (P10587) Pfam (PF02736) Pfam (PF00063) In PDB	Gallus gallus (chicken)	MYOSIN HEAVY CHAIN, GIZZARD SMOOTH MUSCLE
2	T, W	SMOOTH MUSCLE MYOSIN ESSENTIAL LIGHT CHAIN	polymer	150	16873.0	2	GenBank (212393) UniProt (by SIFTS) (P02607) In PDB	Gallus gallus (chicken)	ELC, MYOSIN ALKALI LIGHT CHAIN
3	U, Z	SMOOTH MUSCLE MYOSIN REGULATORY LIGHT CHAIN	polymer	166	18329.6	2	UniProt (P02609) In PDB	Gallus gallus (chicken)	RLC, MYOSIN REGULATORY LIGHT CHAIN 2, SMOOTH MUSCLE (MAJOR)

Sequence modifications

S, V: 2 - 1175 (UniProt: P10587)

PDB	External Database	Details
Ala 2	Ser 1	SEE REMARK 999
Glu 820	Thr 819	SEE REMARK 999
Ser 821	Ala 820	SEE REMARK 999
Ile 822	Met 821	SEE REMARK 999
Phe 823	Lys 822	SEE REMARK 999
Cys 824	Val 823	SEE REMARK 999
Tyr 827	Arg 826	SEE REMARK 999
Val 829	Cys 828	SEE REMARK 999
Arg 830	Ala 829	SEE REMARK 999
Ser 831	Ala 830	SEE REMARK 999
Phe 832	Tyr 831	SEE REMARK 999
Met 833	Leu 832	SEE REMARK 999
Asn 834	Lys 833	SEE REMARK 999
Val 835	Leu 834	SEE REMARK 999
Mly 836	Arg 835	SEE REMARK 999
His 837	Asn 836	SEE REMARK 999
Pro 839	Gln 838	SEE REMARK 999
Met 841	Trp 840	SEE REMARK 999
Mly 842	Arg 841	SEE REMARK 999
Phe 845	Thr 844	SEE REMARK 999
Mly 846	Lys 845	SEE REMARK 999
Ile 847	Val 846	SEE REMARK 999
Mly 848	Lys 847	SEE REMARK 999
Lys 852	Gln 851	SEE REMARK 999
Gly 1176	-	expression tag
Ser 1177	-	expression tag
Asp 1178	-	expression tag
Tyr 1179	-	expression tag
Lys 1180	-	expression tag
Asp 1181	-	expression tag
Asp 1182	-	expression tag
Asp 1183	-	expression tag
Asp 1184	-	expression tag
Lys 1185	-	expression tag

U, Z: 1 - 166 (UniProt: P02609)

PDB	External Database	Details
Glu 21	Gln 21	SEE REMARK 999
Glu 23	Gln 23	SEE REMARK 999
Glu 25	Gln 25	SEE REMARK 999
Asp 26	Glu 26	SEE REMARK 999
Ala 38	Arg 38	SEE REMARK 999
Gly 124	Gln 124	SEE REMARK 999
Gly 125	Cys 125	SEE REMARK 999
Gly 126	Asp 126	SEE REMARK 999
Ala 163	Lys 163	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	344551.1
All*	Total formula weight	344551.1

*Water molecules are not included.