1HL2

Crystal structure of N-acetylneuraminate lyase from E. coli mutant L142R in complex with b-hydroxypyruvate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	N-ACETYLNEURAMINATE LYASE SUBUNIT	polymer	297	32670.4	4	UniProt (P06995) Pfam (PF00701) UniProt (by SIFTS) (P0A6L4)	ESCHERICHIA COLI	N-ACETYLNEURAMINIC ACID ALDOLASE, N-ACETYLNEURAMINATE PYRUVATE LYASE, NALASE
2	E, F, G, H (A, B, C, D)	3-HYDROXYPYRUVIC ACID	non-polymer		104.1	4	Chemie (3PY)
3	I, J, K, L (A, B, C, D)	water	water		18.0	832	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 1 (PDB: 1HL2)
A, B, C, D: 2 - 297 (UniProt: P06995)

PDB	External Database	Details
Arg 142	Leu 141	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	130681.6
Non-Polymers*	Number of molecules	4
	Total formula weight	416.2
All*	Total formula weight	131097.9

*Water molecules are not included.