1H2G
Altered substrate specificity mutant of penicillin acylase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | PENICILLIN G ACYLASE ALPHA SUBUNIT | polymer | 209 | 23838.8 | 1 | UniProt (P06875) | ESCHERICHIA COLI | PENICILLIN AMIDOHYDROLASE, PENICILLIN G AMIDASE |
| 2 | B (B) | PENICILLIN G ACYLASE BETA SUBUNIT | polymer | 557 | 62395.5 | 1 | UniProt (P06875) Pfam (PF01804) | ESCHERICHIA COLI | PENICILLIN AMIDOHYDROLASE, PENICILLIN G AMIDASE |
| 3 | C, E, F, G (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 4 | D (B) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 5 | H, I (A, B) | water | water | 18.0 | 596 | Chemie (HOH) |
Sequence modifications
B: 1 - 557 (UniProt: P06875)
| PDB | External Database | Details |
|---|---|---|
| Leu 71 | Phe 360 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 86234.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 288.4 | |
| All* | Total formula weight | 86522.7 |
