1GS3
High resolution crystal structure of PI delta-5-3-Ketosteroid Isomerase mutants Y30F/Y55F/Y115F/D38N (Y32F/Y57F/Y119F/D40N, PI numbering)complexed with equilenin at 2.1 A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | STEROID DELTA-ISOMERASE | polymer | 131 | 14499.5 | 1 | UniProt (P07445) Pfam (PF12680) | PSEUDOMONAS PUTIDA | DELTA-5-3-KETOSTEROID ISOMERASE |
| 2 | B (A) | EQUILENIN | non-polymer | 266.3 | 1 | Chemie (EQU) | |||
| 3 | C (A) | water | water | 18.0 | 38 | Chemie (HOH) |
Sequence modifications
A: 1 - 131 (UniProt: P07445)
| PDB | External Database | Details |
|---|---|---|
| Phe 32 | Tyr 32 | engineered mutation |
| Asn 40 | Asp 40 | engineered mutation |
| Phe 57 | Tyr 57 | engineered mutation |
| Phe 119 | Tyr 119 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 14499.5 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 266.3 | |
| All* | Total formula weight | 14765.8 |
