1GM8
Crystal structures of penicillin acylase enzyme-substrate complexes: Structural insights into the catalytic mechanism
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | PENICILLIN G ACYLASE ALPHA SUBUNIT | polymer | 209 | 23838.8 | 1 | UniProt (P06875) | ESCHERICHIA COLI | NTN, PENICILLIN AMIDOHYDROLASE |
| 2 | B (B) | PENICILLIN G ACYLASE BETA SUBUNIT | polymer | 557 | 62386.5 | 1 | UniProt (P06875) Pfam (PF01804) | ESCHERICHIA COLI | NTN, PENICILLIN AMIDOHYDROLASE |
| 3 | C (B) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | D (B) | N-[(2S,4S,6R)-2-(DIHYDROXYMETHYL)-4-HYDROXY-3,3-DIMETHYL-7-OXO-4LAMBDA~4~-THIA-1-AZABICYCLO[3.2.0]HEPT-6-YL]-2-PHENYLAC ETAMIDE | non-polymer | 352.4 | 1 | Chemie (SOX) | |||
| 5 | E, F (A, B) | water | water | 18.0 | 644 | Chemie (HOH) |
Sequence modifications
B: 1 - 557 (UniProt: P06875)
| PDB | External Database | Details |
|---|---|---|
| Ala 241 | Asn 530 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 86225.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 392.5 | |
| All* | Total formula weight | 86617.8 |
