1GK1
Structure-based prediction of modifications in glutarylamidase to allow single-step enzymatic production of 7-aminocephalosporanic acid from cephalosporin C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | CEPHALOSPORIN ACYLASE | polymer | 153 | 16941.6 | 2 | UniProt (O86089) UniProt (by SIFTS) (P07662) | PSEUDOMONAS SP. | GLUTARYLAMIDASE |
| 2 | B, D (B, D) | CEPHALOSPORIN ACYLASE | polymer | 522 | 58339.9 | 2 | UniProt (O86089) Pfam (PF01804) UniProt (by SIFTS) (P07662) | PSEUDOMONAS SP. | GLUTARYLAMIDASE |
| 3 | E, F (B, D) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 4 | G, H, I, J (A, B, C, D) | water | water | 18.0 | 659 | Chemie (HOH) |
Sequence modifications
A, C: 8 - 160 (UniProt: O86089)
| PDB | External Database | Details |
|---|---|---|
| Glu 126 | Asp 154 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 150563.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 184.2 | |
| All* | Total formula weight | 150747.3 |
