1GES

ANATOMY OF AN ENGINEERED NAD-BINDING SITE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	GLUTATHIONE REDUCTASE	polymer	450	48739.1	2	UniProt (P06715) Pfam (PF00070) Pfam (PF02852) In PDB	Escherichia coli
2	A, B	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3		water	water		18.0	585	Chemie (HOH)

Sequence modifications

A, B: 1 - 450 (UniProt: P06715)

PDB	External Database	Details
Gly 179	Ala 179	engineered mutation
Gly 183	Ala 183	engineered mutation
Glu 197	Val 197	engineered mutation
Met 198	Arg 198	engineered mutation
Phe 199	Lys 199	engineered mutation
Asp 200	His 200	engineered mutation
Pro 204	Arg 204	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97478.3
Non-Polymers*	Number of molecules	2
	Total formula weight	1571.1
All*	Total formula weight	99049.4

*Water molecules are not included.