1EZS

CRYSTAL STRUCTURE OF ECOTIN MUTANT M84R, W67A, G68A, Y69A, D70A BOUND TO RAT ANIONIC TRYPSIN II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ECOTIN	polymer	142	15909.3	2	UniProt (P23827) Pfam (PF03974)	Escherichia coli
2	C, D (C, D)	TRYPSIN II, ANIONIC	polymer	223	23814.8	2	UniProt (P00763) Pfam (PF00089)	Rattus norvegicus (Norway rat)
3	E, F (C, D)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	G, H, I, J (A, B, C, D)	water	water		18.0	86	Chemie (HOH)

Sequence modifications

A, B: 1 - 142 (UniProt: P23827)

PDB	External Database	Details
Ala 67	Trp 87	engineered mutation
Ala 68	Gly 88	engineered mutation
Ala 69	Tyr 89	engineered mutation
Ala 70	Asp 90	engineered mutation
Arg 84	Met 104	engineered mutation

C, D: 416 - 645 (UniProt: P00763)

PDB	External Database	Details
Asp 479	Asn 84	SEE REMARK 999
Asn 502	Asp 107	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	79448.3
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	79528.4

*Water molecules are not included.