1EVW

L116A MUTANT OF THE HOMING ENDONUCLEASE I-PPOI COMPLEXED TO HOMING SITE DNA.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (E, G)	DNA (5'-D(*TP*GP*GP*CP*TP*AP*CP*CP*TP*TP*AP*A)-3')	polymer	12	3637.4	2
2	B, F (M, N)	DNA (5'-D(P*GP*AP*GP*AP*GP*TP*CP*A)-3')	polymer	8	2475.7	2
3	C, G (F, H)	DNA (5'-D(*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*A)-3')	polymer	12	3612.4	2
4	D, H (O, P)	DNA (5'-D(P*GP*GP*TP*AP*GP*CP*CP*A)-3')	polymer	8	2451.6	2
5	I, J, K, L (A, B, C, D)	I-PPOI HOMING ENDONUCLEASE	polymer	163	17769.2	4	UniProt (Q94702) Pfam (PF05551)	Physarum polycephalum	I-PPOI, INTRON-ENCODED ENDONUCLEASE I-PPOI
6	M, N, Q, T (N, P, A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	O, P, R, S, U... (A, B, C, D)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)

Sequence modifications

A, B, C, D: 1 - 163 (UniProt: Q94702)

PDB	External Database	Details
Ala 116	Leu 116	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	95430.8
Non-Polymers*	Number of molecules	12
	Total formula weight	620.5
All*	Total formula weight	96051.2

*Water molecules are not included.