1EVW

L116A MUTANT OF THE HOMING ENDONUCLEASE I-PPOI COMPLEXED TO HOMING SITE DNA.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E, G	DNA (5'-D(*TP*GP*GP*CP*TP*AP*CP*CP*TP*TP*AP*A)-3')	polymer	12	3637.4	2
2	M, N	DNA (5'-D(P*GP*AP*GP*AP*GP*TP*CP*A)-3')	polymer	8	2475.7	2
3	F, H	DNA (5'-D(*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*A)-3')	polymer	12	3612.4	2
4	O, P	DNA (5'-D(P*GP*GP*TP*AP*GP*CP*CP*A)-3')	polymer	8	2451.6	2
5	A, B, C, D	I-PPOI HOMING ENDONUCLEASE	polymer	163	17769.2	4	UniProt (Q94702) Pfam (PF05551) In PDB	Physarum polycephalum	I-PPOI, INTRON-ENCODED ENDONUCLEASE I-PPOI
6	N, P, A, B	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	A, B, C, D	ZINC ION	non-polymer		65.4	8	Chemie (ZN)

Sequence modifications

A, B, C, D: 1 - 163 (UniProt: Q94702)

PDB	External Database	Details
Ala 116	Leu 116	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	95430.8
Non-Polymers*	Number of molecules	12
	Total formula weight	620.5
All*	Total formula weight	96051.2

*Water molecules are not included.