1EB9

Structure Determinants of Substrate Specificity of Hydroxynitrile Lyase from Manihot esculenta

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	HYDROXYNITRILE LYASE	polymer	262	29720.1	2	UniProt (P52705) Pfam (PF00561) In PDB	MANIHOT ESCULENTA (CASSAVA)	(S)-HYDROXYNITRILE LYASE, (S)-HYDROXYNITRILASE, OXYNITRILASE, HYDROXYNITRILE LYASE
2	A, B	P-HYDROXYBENZALDEHYDE	non-polymer		122.1	4	Chemie (HBA)
3		water	water		18.0	271	Chemie (HOH)

Sequence modifications

A, B: -4 - 1 (PDB: 1EB9)
A, B: 2 - 258 (UniProt: P52705)

PDB	External Database	Details
Ala 128	Trp 127	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59440.2
Non-Polymers*	Number of molecules	4
	Total formula weight	488.5
All*	Total formula weight	59928.6

*Water molecules are not included.