1E8D
MECHANISTIC ASPECTS OF CYANOGENESIS FROM ACTIVE SITE MUTANT SER80ALA OF HYDROXYNITRILE LYASE FROM MANIHOT ESCULENTA IN COMPLEX WITH ACETONE CYANOHYDRIN
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | HYDROXYNITRILE LYASE | polymer | 262 | 29819.2 | 2 | UniProt (P52705) Pfam (PF00561) In PDB | MANIHOT ESCULENTA (CASSAVA) | (S)-ACETONE-CYANOHYDRIN LYASE, (S)-HYDROXYNITRILASE |
| 2 | A, B | 2-HYDROXY-2-METHYLPROPANENITRILE | non-polymer | 85.1 | 4 | Chemie (CNH) | |||
| 3 | water | water | 18.0 | 273 | Chemie (HOH) |
Sequence modifications
A, B: -4 - 1 (PDB: 1E8D)
A, B: 2 - 258 (UniProt: P52705)
A, B: 2 - 258 (UniProt: P52705)
| PDB | External Database | Details |
|---|---|---|
| Pro -4 | - | cloning artifact |
| Ile -3 | - | cloning artifact |
| Ser -2 | - | cloning artifact |
| Lys -1 | - | cloning artifact |
| Met 1 | - | cloning artifact |
| Ala 80 | Ser 79 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 59638.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 340.4 | |
| All* | Total formula weight | 59978.8 |
