1DED
CRYSTAL STRUCTURE OF ALKALOPHILIC ASPARAGINE 233-REPLACED CYCLODEXTRIN GLUCANOTRANSFERASE COMPLEXED WITH AN INHIBITOR, ACARBOSE, AT 2.0 A RESOLUTION
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | CYCLODEXTRIN GLUCANOTRANSFERASE | polymer | 686 | 75206.0 | 2 | UniProt (P05618) Pfam (PF00128) Pfam (PF02806) Pfam (PF01833) Pfam (PF00686) In PDB | Bacillus sp. | CGTASE |
| 2 | C, D, E | 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 645.6 | 3 | In PDB BIRD (PRD_900022) GlyTouCan (G08743RW) | beta-acarbose | ||
| 3 | A, B | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 4 | water | water | 18.0 | 759 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 686 (UniProt: P05618)
| PDB | External Database | Details |
|---|---|---|
| Asn 233 | His 260 | engineered mutation |
| Pro 452 | Arg 479 | conflict |
| Gly 454 | Ala 481 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 150412.1 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1936.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 160.3 | |
| All* | Total formula weight | 152509.2 |
