13CP

Cryo-EM structure of an intact human acetylcholinesterase (T-form) tetramer in complex with PRiMA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Acetylcholinesterase	polymer	583	64641.7	4	UniProt (P22303) Pfam (PF00135) Pfam (PF08674)	Homo sapiens (human)	AChE
2	E (E)	Small ubiquitin-related modifier,Proline-rich membrane anchor 1	polymer	175	19868.1	1	UniProt (Q12306) UniProt (Q86XR5) Pfam (PF00240) Pfam (PF16101)	Homo sapiens (human)	SUMO,Suppressor of mif two,Ubiquitin-like protein SMT3,PRiMA
3	F, G, H, I (F, G, H, I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	4	In PDB GlyTouCan (G21290RB)
4	J, K, L, M, N... (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	8	Chemie (NAG) PubChem (24139)

Sequence modifications

E: -102 - -7 (UniProt: Q12306)

PDB	External Database	Details
Glu -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
His -114	-	expression tag
His -113	-	expression tag
His -112	-	expression tag
His -111	-	expression tag
His -110	-	expression tag
His -109	-	expression tag
His -108	-	expression tag
His -107	-	expression tag
Gly -106	-	expression tag
Ser -105	-	expression tag
Leu -104	-	expression tag
Gln -103	-	expression tag
Thr -41	Arg 64	engineered mutation
Glu -34	Arg 71	engineered mutation
Glu -6	-	linker
Asn -5	-	linker
Leu -4	-	linker
Tyr -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Ser 0	-	linker

E: 1 - 57 (UniProt: Q86XR5)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	278435.1
Branched	Number of molecules	4
	Total formula weight	2282.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1769.7
All*	Total formula weight	282486.9

*Water molecules are not included.