12ZN

Septin tetrameric complex from Caenorhabditis elegans by Cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Septin	polymer	462	53135.7	2	UniProt (Q9U277)	Caenorhabditis elegans
2	B, C (B, C)	Septin	polymer	478	55299.5	2	UniProt (Q9U334)	Caenorhabditis elegans
3	E, F (B, C)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)

Sequence modifications

A, D: 1 - 461 (UniProt: Q9U277)

PDB	External Database	Details
Met 0	-	initiating methionine

B, C: 1 - 458 (UniProt: Q9U334)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	216870.4
Non-Polymers*	Number of molecules	2
	Total formula weight	886.4
All*	Total formula weight	217756.8

*Water molecules are not included.