12SN

SARS-CoV-2 RNA-dependent RNA polymerase in complex with 4'-FlA nucleotide analogue

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA-directed RNA polymerase nsp12	polymer	975	111110.6	1	UniProt (P0DTD1)	Severe acute respiratory syndrome coronavirus 2	Pol,RdRp,Non-structural protein 12,nsp12
2	B, D (B, D)	Non-structural protein 8	polymer	217	24192.5	2	UniProt (P0DTD1)	Severe acute respiratory syndrome coronavirus 2	nsp8
3	C (C)	Non-structural protein 7	polymer	102	11538.3	1	UniProt (P0DTC1)	Severe acute respiratory syndrome coronavirus 2	nsp7
4	E (P)	RNA (5'-R(P*GP*AP*UP*UP*AP*AP*GP*UP*UP*AP*U)-3')	polymer	20	6410.8	1		Severe acute respiratory syndrome coronavirus 2
5	F (T)	RNA (5'-R(P*UP*AP*UP*AP*AP*CP*UP*UP*AP*AP*UP*C)-3')	polymer	30	9358.5	1		Severe acute respiratory syndrome coronavirus 2
6	G, H (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)
7	I (A)	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
8	J (A)	[(2S,3S,4R,5R)-5-(4-amino-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-2-fluoro-3,4-dihydroxyoxolan-2-yl]methyl dihydrogen phosphate	non-polymer		364.2	1	Chemie (A1DCZ)
9	K (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

A: 1 - 932 (UniProt: P0DTD1)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 933	-	expression tag
Gly 934	-	expression tag
Ser 935	-	expression tag
Glu 936	-	expression tag
Asn 937	-	expression tag
Leu 938	-	expression tag
Tyr 939	-	expression tag
Phe 940	-	expression tag
Gln 941	-	expression tag
Gly 942	-	expression tag
Gly 943	-	expression tag
Gly 944	-	expression tag
Ser 945	-	expression tag
Ala 946	-	expression tag
Trp 947	-	expression tag
Ser 948	-	expression tag
His 949	-	expression tag
Pro 950	-	expression tag
Gln 951	-	expression tag
Phe 952	-	expression tag
Glu 953	-	expression tag
Lys 954	-	expression tag
Gly 955	-	expression tag
Gly 956	-	expression tag
Gly 957	-	expression tag
Ser 958	-	expression tag
Gly 959	-	expression tag
Gly 960	-	expression tag
Gly 961	-	expression tag
Ser 962	-	expression tag
Gly 963	-	expression tag
Gly 964	-	expression tag
Ser 965	-	expression tag
Ala 966	-	expression tag
Trp 967	-	expression tag
Ser 968	-	expression tag
His 969	-	expression tag
Pro 970	-	expression tag
Gln 971	-	expression tag
Phe 972	-	expression tag
Glu 973	-	expression tag
Lys 974	-	expression tag

B, D: 1 - 198 (UniProt: P0DTD1)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 199	-	expression tag
Gly 200	-	expression tag
Ser 201	-	expression tag
Glu 202	-	expression tag
Asn 203	-	expression tag
Leu 204	-	expression tag
Tyr 205	-	expression tag
Phe 206	-	expression tag
Gln 207	-	expression tag
Gly 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag
His 216	-	expression tag

C: 1 - 83 (UniProt: P0DTC1)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 84	-	expression tag
Gly 85	-	expression tag
Ser 86	-	expression tag
Glu 87	-	expression tag
Asn 88	-	expression tag
Leu 89	-	expression tag
Tyr 90	-	expression tag
Phe 91	-	expression tag
Gln 92	-	expression tag
Gly 93	-	expression tag
His 94	-	expression tag
His 95	-	expression tag
His 96	-	expression tag
His 97	-	expression tag
His 98	-	expression tag
His 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	186803.1
Non-Polymers*	Number of molecules	5
	Total formula weight	695.3
All*	Total formula weight	187498.4

*Water molecules are not included.