12JA
Cryo-EM structure of human cannabinoid receptor 2-Gi complex with agonist '1029
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 350 | 38534.1 | 1 | UniProt (P62873) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) | Homo sapiens (human) | G gamma-I |
| 4 | D (R) | Cannabinoid receptor 2 | polymer | 374 | 41442.4 | 1 | UniProt (P34972) | Homo sapiens (human) | CB-2,CB2,hCB2,CX5 |
| 5 | E (R) | methyl N-[4-ethyl-3-(morpholine-4-sulfonyl)benzoyl]-N-hexylglycinate | non-polymer | 454.6 | 1 | Chemie (A1DB6) | |||
| 6 | F (R) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
R: 1 - 360 (UniProt: P34972)
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp -13 | - | expression tag |
| Tyr -12 | - | expression tag |
| Lys -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Asn -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 128251.6 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 454.6 | |
| All* | Total formula weight | 128706.2 |
