12CE

Head-on RNAP-RNAP Collision: 26 bp distance

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (N)	Non-template strand homopolymer	polymer	34	10707.8	1		synthetic construct
2	B, C (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4)	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
3	D (C)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2)	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
4	E (D)	DNA-directed RNA polymerase subunit beta'	polymer	1415	156306.8	1	UniProt (P0A8T7)	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
5	F (E)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800)	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
6	G (R)	RNA strand	polymer	13	4442.7	1		synthetic construct
7	H (T)	Template strand homopolymer	polymer	33	9498.0	1		synthetic construct
8	I, J (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)
9	K (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

D: 2 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Val 1	-	expression tag
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Leu 1410	-	expression tag
Glu 1411	-	expression tag
Val 1412	-	expression tag
Leu 1413	-	expression tag
Phe 1414	-	expression tag
Gln 1415	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	415143.1
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	415298.3

*Water molecules are not included.