12CD
RNAP-EcoRI* Collision
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 3 | D, E (F, G) | Type II restriction enzyme EcoRI | polymer | 277 | 31100.4 | 2 | UniProt (P00642) | Escherichia coli | R.EcoRI,Endonuclease EcoRI,Type-2 restriction enzyme EcoRI |
| 4 | F (N) | Non-template strand | polymer | 76 | 23374.0 | 1 | synthetic construct | ||
| 5 | G (R) | RNA strand | polymer | 43 | 13729.2 | 1 | synthetic construct | ||
| 6 | H (T) | Template strand homopolymer | polymer | 76 | 23490.1 | 1 | synthetic construct | ||
| 7 | I (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 8 | J (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1415 | 156306.8 | 1 | UniProt (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 9 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) | |||
| 10 | M (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) |
Sequence modifications
F, G: 1 - 277 (UniProt: P00642)
D: 2 - 1407 (UniProt: P0A8T7)
| PDB | External Database | Details |
|---|---|---|
| Gln 111 | Glu 111 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Val 1 | - | expression tag |
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| Leu 1410 | - | expression tag |
| Glu 1411 | - | expression tag |
| Val 1412 | - | expression tag |
| Leu 1413 | - | expression tag |
| Phe 1414 | - | expression tag |
| Gln 1415 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 513288.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 513443.8 |
