12BP

Cryo-EM structure of human DDB1-CRBN-GSPT1 in complex with GT19630

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (X)	Eukaryotic peptide chain release factor GTP-binding subunit ERF3A	polymer	218	24154.2	1	UniProt (P15170)	Homo sapiens (human)	Eukaryotic peptide chain release factor subunit 3a,eRF3a,G1 to S phase transition protein 1 homolog
2	B (Z)	Protein cereblon	polymer	406	46653.6	1	UniProt (Q96SW2)	Homo sapiens (human)
3	C (Y)	DNA damage-binding protein 1	polymer	836	93347.1	1	UniProt (Q16531)	Homo sapiens (human)	DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe
4	D (Z)	2-[(3S)-2,6-dioxopiperidin-3-yl]-1-oxo-N-[(4-{[2-(9H-pyrido[2,3-b]indol-9-yl)acetamido]methyl}phenyl)methyl]-2,3-dihydro-1H-isoindole-5-carboxamide	non-polymer		614.6	1	Chemie (A1DBR)
5	E (Z)	ZINC ION	non-polymer		65.4	1	Chemie (ZN) PubChem (32051)

Sequence modifications

X: 438 - 634 (UniProt: P15170)

PDB	External Database	Details
Met 417	-	expression tag
Gly 418	-	expression tag
Ser 419	-	expression tag
Ser 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag
His 426	-	expression tag
Ser 427	-	expression tag
Ser 428	-	expression tag
Gly 429	-	expression tag
Leu 430	-	expression tag
Val 431	-	expression tag
Pro 432	-	expression tag
Arg 433	-	expression tag
Gly 434	-	expression tag
Ser 435	-	expression tag
His 436	-	expression tag
Met 437	-	expression tag

Z: 40 - 442 (UniProt: Q96SW2)

PDB	External Database	Details
Ser 37	-	expression tag
Asn 38	-	expression tag
Met 39	-	expression tag

Y: 1 - 699 (UniProt: Q16531)
Y: 706 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Gly 700	-	linker
Asn 701	-	linker
Gly 702	-	linker
Asn 703	-	linker
Ser 704	-	linker
Gly 705	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	164154.9
Non-Polymers*	Number of molecules	2
	Total formula weight	680.1
All*	Total formula weight	164835.0

*Water molecules are not included.