11ZK

Cryo-EM structure of NifI free Nitrogenase MoFe protein from Methanosarcina acetivorans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, A)	Nitrogenase protein alpha chain	polymer	559	62884.3	2	UniProt (Q8TJ90) Pfam (PF00148)	Methanosarcina acetivorans
2	C, D (B, D)	Nitrogenase molybdenum-iron protein beta chain	polymer	456	49478.6	2	UniProt (Q8TJ89) Pfam (PF00148)	Methanosarcina acetivorans	Dinitrogenase
3	E (A)	iron-sulfur-molybdenum cluster with interstitial carbon	non-polymer		787.5	1	Chemie (ICS)
4	F (D)	FE(8)-S(7) CLUSTER	non-polymer		671.2	1	Chemie (CLF)

Sequence modifications

C, A: 1 - 528 (UniProt: Q8TJ90)

PDB	External Database	Details
Met -30	-	initiating methionine
Trp -29	-	expression tag
Ser -28	-	expression tag
His -27	-	expression tag
Pro -26	-	expression tag
Gln -25	-	expression tag
Phe -24	-	expression tag
Glu -23	-	expression tag
Lys -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Trp -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
Pro -6	-	expression tag
Gln -5	-	expression tag
Phe -4	-	expression tag
Glu -3	-	expression tag
Lys -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	224725.8
Non-Polymers*	Number of molecules	2
	Total formula weight	1458.7
All*	Total formula weight	226184.4

*Water molecules are not included.