11TA
Cryo-EM structure of substrate engaged p97-Ufd1-NPL4-Faf1 complex (motor focused)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (G) | Nuclear protein localization protein 4 homolog | polymer | 611 | 68373.2 | 1 | UniProt (Q8TAT6) Pfam (PF11543) Pfam (PF05020) Pfam (PF05021) | Homo sapiens (human) | Protein NPL4 |
| 2 | B, C (I, K) | Ubiquitin | polymer | 76 | 8576.8 | 2 | UniProt (P62987) Pfam (PF00240) | Homo sapiens (human) | |
| 3 | D, E (M, O) | FAS-associated factor 1 | polymer | 173 | 20495.0 | 2 | UniProt (Q9UNN5) Pfam (PF00789) | Homo sapiens (human) | hFAF1,UBX domain-containing protein 12,UBX domain-containing protein 3A |
| 4 | F (P) | Ubiquitin recognition factor in ER-associated degradation protein 1 | polymer | 313 | 35373.2 | 1 | UniProt (Q92890) Pfam (PF03152) Pfam (PF24842) | Homo sapiens (human) | Ubiquitin fusion degradation protein 1,UB fusion protein 1 |
| 5 | G, H, I, J, K... (A, B, C, D, E...) | Transitional endoplasmic reticulum ATPase | polymer | 821 | 91177.8 | 6 | UniProt (P55072) Pfam (PF02359) Pfam (PF02933) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (human) | TER ATPase,15S Mg(2+)-ATPase p97 subunit,Valosin-containing protein,VCP |
| 6 | M, N (G) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) | |||
| 7 | O, Q, S (A, B, C) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 3 | Chemie (ATP) | |||
| 8 | P, R, T, U, V... (A, B, C, D, E...) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 9 | Chemie (ADP) |
Sequence modifications
G: 2 - 608 (UniProt: Q8TAT6)
M, O: 481 - 650 (UniProt: Q9UNN5)
P: 1 - 307 (UniProt: Q92890)
A, B, C, D, E, F: 1 - 806 (UniProt: P55072)
| PDB | External Database | Details |
|---|---|---|
| Met -2 | - | initiating methionine |
| Gly -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 478 | - | expression tag |
| Leu 479 | - | expression tag |
| Gly 480 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| His 308 | - | expression tag |
| His 309 | - | expression tag |
| His 310 | - | expression tag |
| His 311 | - | expression tag |
| His 312 | - | expression tag |
| His 313 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Val 807 | - | expression tag |
| Asp 808 | - | expression tag |
| Lys 809 | - | expression tag |
| Leu 810 | - | expression tag |
| Ala 811 | - | expression tag |
| Ala 812 | - | expression tag |
| Ala 813 | - | expression tag |
| Leu 814 | - | expression tag |
| Glu 815 | - | expression tag |
| His 816 | - | expression tag |
| His 817 | - | expression tag |
| His 818 | - | expression tag |
| His 819 | - | expression tag |
| His 820 | - | expression tag |
| His 821 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 708956.7 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 5497.2 | |
| All* | Total formula weight | 714453.9 |
