11SY

Cryo-EM structure of substrate engaged p97-Ufd1-NPL4-Faf1 complex (NPL4 focused)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (G)	Nuclear protein localization protein 4 homolog	polymer	611	68373.2	1	UniProt (Q8TAT6) Pfam (PF11543) Pfam (PF05020) Pfam (PF05021)	Homo sapiens (human)	Protein NPL4
2	B, C, D, E (H, I, J, K)	Ubiquitin	polymer	76	8576.8	4	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (human)
3	F (P)	Ubiquitin recognition factor in ER-associated degradation protein 1	polymer	313	35373.2	1	UniProt (Q92890) Pfam (PF03152) Pfam (PF24842)	Homo sapiens (human)	Ubiquitin fusion degradation protein 1,UB fusion protein 1
4	G, H (G)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)

Sequence modifications

G: 2 - 608 (UniProt: Q8TAT6)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag

P: 1 - 307 (UniProt: Q92890)

PDB	External Database	Details
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	138053.7
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	138184.5

*Water molecules are not included.