11SX

Cryo-EM structure of trimeric Nitrogenase MoFe protein from Methanosarcina acetivorans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Nitrogenase protein alpha chain	polymer	559	62884.3	1	UniProt (Q8TJ90) Pfam (PF00148)	Methanosarcina acetivorans
2	B, C (B, D)	Nitrogenase molybdenum-iron protein beta chain	polymer	456	49478.6	2	UniProt (Q8TJ89) Pfam (PF00148)	Methanosarcina acetivorans	Dinitrogenase
3	D (A)	iron-sulfur-molybdenum cluster with interstitial carbon	non-polymer		787.5	1	Chemie (ICS)
4	E (B)	FE(8)-S(7) CLUSTER	non-polymer		671.2	1	Chemie (CLF)

Sequence modifications

A: 1 - 528 (UniProt: Q8TJ90)

PDB	External Database	Details
Met -30	-	initiating methionine
Trp -29	-	expression tag
Ser -28	-	expression tag
His -27	-	expression tag
Pro -26	-	expression tag
Gln -25	-	expression tag
Phe -24	-	expression tag
Glu -23	-	expression tag
Lys -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Trp -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
Pro -6	-	expression tag
Gln -5	-	expression tag
Phe -4	-	expression tag
Glu -3	-	expression tag
Lys -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	161841.5
Non-Polymers*	Number of molecules	2
	Total formula weight	1458.7
All*	Total formula weight	163300.2

*Water molecules are not included.